【SCI通讯】Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses
- 所属单位：化学与生命科学学院
- 教研室：化学与生命科学学院
- 发表刊物：Journal of Nanobiotechnology
- 项目来源：省、市、自治区科技项目
- 摘要：Abstract Background: Canine distemper virus (CDV), which is highly infectious, has caused outbreaks of varying scales in domestic and wild animals worldwide, so the development of a high-efciency vaccine has broad application prospects. Currently, the commercial vaccine of CDV is an attenuated vaccine, which has the disadvantages of a complex preparation process, high cost and safety risk. It is necessary to develop a safe and efective CDV vaccine that is easy to produce on a large scale. In this study, sequences of CDV haemagglutinin (HA) from the Yanaka strain were aligned, and three potential linear sequences, termed YaH3, YaH4, and YaH5, were collected. To increase the immunogenicity of the epitopes, ferritin was employed as a self-assembling nanoparticle element. The ferritin-coupled forms were termed YaH3F, YaH4F, and YaH5F, respectively. A full-length HA sequence coupled with ferritin was also constructed as a DNA vaccine to compare the immunogenicity of nanoparticles in prokaryotic expression. Result: The self-assembly morphology of the proteins from prokaryotic expression was verifed by transmission electron microscopy. All the proteins self-assembled into nanoparticles. The expression of the DNA vaccine YaHF in HEK-293T cells was also confrmed in vitro. After subcutaneous injection of epitope nanoparticles or intramuscular injection of DNA YaHF, all vaccines induced strong serum titres, and long-term potency of antibodies in serum could be detected after 84 days. Strong anti-CDV neutralizing activities were observed in both the YaH4F group and YaHF group. According to antibody typing and cytokine detection, YaH4F can induce both Th1 and Th2 immune responses. The results of fow cytometry detection indicated that compared with the control group, all the immunogens elicited an increase in CD3. Simultaneously, the serum antibodies induced by YaH4F and YaHF could signifcantly enhance the ADCC efect compared with the control group, indicating that the antibodies in the serum efectively recognized the antigens on the cell surface and induced NK cells to kill infected cells directly. Conclusions: YaH4F self-assembling nanoparticle obtained by prokaryotic expression has no less of an immune efect than YaHF, and H4 has great potential to become a key target for the easy and rapid preparation of epitope vaccines
- 第一作者：殷玉和
- 论文类型：期刊论文
- 页面范围：1
- 是否译文：否
- 发表时间：2022-01-11
