【SCI通讯】Self-assembled raccoon dog parvovirus VP2 protein confers immunity against RDPV disease in raccoon dogs:in vitro and in vivo studies
- 所属单位：化学与生命科学学院
- 教研室：化学与生命科学学院
- 发表刊物：Viroiogy Journal
- 项目来源：省、市、自治区科技项目
- 关键字：Raccoon dog parvovirus, Virus like particle, Trigger factor 16, Escherichia coli, Immunogenicity
- 摘要：Abstract Background: Raccoon dog parvovirus (RDPV) causes acute infectious diseases in raccoon dogs and may cause death in severe cases. The current treatment strategy relies on the extensive usage of classical inactivated vaccine which is marred by large doses, short immunization cycles and safety concerns. Methods: The present study aimed at optimization of RDPV VP2 gene, subcloning the gene into plasmid pET30a, and its subsequent transfer to Escherichia coli with trigger factor 16 for co-expression. The protein thus expressed was purifed with ammonium sulfate precipitation, hydrophobic chromatography, and endotoxin extraction procedures. VLPs were examined by transmission electron microscopy, dynamic light scattering, and the efcacy of VLPs vaccine was tested in vivo. Results: Results indicated that RDPV VP2 protein could be expressed soluble. Transmission electron microscopy and dynamic light scattering results indicated that RDPV VP2 self-assembled into VLPs. Hemagglutination inhibition antibody titers elicited by Al(OH)3 adjuvanted RDPV VLPs were comparable with RDPV inactivated vaccines, and the viral loads in the blood of the struck raccoon dogs were greatly reduced. Hematoxylin and eosin and Immunohistochemical results indicated that RDPV VLPs vaccine could protect raccoon dogs against RDPV infections. Conclusions: These results suggest that RDPV VLPs can become a potential vaccine candidate for RDPV therapy
- 合写作者：夏霖亚
- 第一作者：殷玉和
- 论文类型：期刊论文
- 页面范围：1
- 是否译文：否
- 发表时间：2021-04-15
