所属单位：化学与生命科学学院

教研室：生物工程教研室

发表刊物：Bulgarian Chemical Communications

项目来源：自选课题

关键字：Voriconazole; LC-MS/MS; Human plasma

摘要：rapid and sensitive method for the determination of voriconazole in human plasma was developed. Voriconazole and the internal standard were extracted from plasma samples by liquid–liquid extraction with 2 ml of diethyl ether: diethyl ether: diethyl ether: diethyl ether: diethyl ether: diethyl ether: diethyl ether: diethyl ether: diethyl ether: diethyl ether: dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v) dichloromethane (60: 40, v/v) dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v) dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v) dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v) dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v)dichloromethane (60: 40, v/v) dichloromethane (60: 40, v/v). The chromatographic separation was accomplished isocratically on a 150×4.6 mm, 5 m Zorbax extend C18 column at a flow rate of 0.7 mL/min. Detec Detection by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass tion by electrospray positive ionization mass spectrometry in the multiplespectrometry in the multiplespectrometry in the multiple spectrometry in the multiple spectrometry in the multiplespectrometry in the multiplespectrometry in the multiplespectrometry in the multiple spectrometry in the multiplespectrometry in the multiplespectrometry in the multiplespectrometry in the multiplespectrometry in the multiplespectrometry in the multiple spectrometry in the multiple spectrometry in the multiplespectrometry in the multiplespectrometry in the multiple spectrometry in the multiple -reaction monitoring modereaction monitoring mode reaction monitoring mode reaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring mode reaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring modereaction monitoring mode was completed within 3.2 min. Linearity was over the the the the concentration range concentration range concentration range concentration range concentration range concentration range concentration range concentration range concentration range concentration range concentration range concentration range 20-2000 ng/ml with ng/ml with ng/ml with ng/ml with ng/ml with ng/ml with ng/ml with ng/ml with ng/ml with a limit of detection limit of detection oflimit of detection limit of detection oflimit of detection oflimit of detection oflimit of detection oflimit of detection limit of detection limit of detection oflimit of detection oflimit of detection oflimit of detection oflimit of detection 5 ng/mlng/mlng/mlng/mlng/ml. Intra- and inter-day precision measured as relative standard deviation was <4.40% and <5.44%, respectively. The method was applied in a bioequivalence study of two tablet formulations of voriconazole.

第一作者：滕国生

论文类型：期刊论文

卷号：Volume 48

期号：Number 1

页面范围：1

ISSN号：0324-1130

是否译文：否

发表时间：2016-01-10